rabbit polyclonal antibody against timp1  (Proteintech)

Journal: Experimental and molecular pathology

Article Title: Intracerebral matrix metalloproteinase 9 in fatal diabetic ketoacidosis

doi: 10.1016/j.yexmp.2019.04.008

Figure Lengend Snippet: Expression of MMP9 and TIMP1 in DKA and normal brains −, negative; +, slightly positive; ++, positive; +++, highly positive; ND, not determined.

Article Snippet: Sections were preincubated for 30 min with normal goat serum then with a rabbit monoclonal antibody (mAb) against human MMP9 (CST, Danvers, MA) or a rabbit polyclonal antibody against human TIMP1 (Millipore Sigma, Burlington, MA) over-night at 4°C in a humid chamber.

Techniques: Expressing

Journal: Experimental and molecular pathology

Article Title: Intracerebral matrix metalloproteinase 9 in fatal diabetic ketoacidosis

doi: 10.1016/j.yexmp.2019.04.008

Figure Lengend Snippet: The percentage of MMP9+ (A) and TIMP1+ (B) hippocampal neurons was determined by counting 200 cells from 3 controls and 2 DKA brains. We did not find MMP9+ neurons in control brains (A) and an arbitrary value of 1 was given. There was a statistically significant increase of MMP9+ neurons in DKA brains as compared to control brains (p=0.002). In addition, we found significantly higher levels of TIMP1 expression in neurons in the control brains as compared to DKA brains (p=0.03) (B). Data are shown as mean ± SEM.

Article Snippet: Sections were preincubated for 30 min with normal goat serum then with a rabbit monoclonal antibody (mAb) against human MMP9 (CST, Danvers, MA) or a rabbit polyclonal antibody against human TIMP1 (Millipore Sigma, Burlington, MA) over-night at 4°C in a humid chamber.

Techniques: Expressing

Journal: Experimental and molecular pathology

Article Title: Intracerebral matrix metalloproteinase 9 in fatal diabetic ketoacidosis

doi: 10.1016/j.yexmp.2019.04.008

Figure Lengend Snippet: TIMP1 was localized by indirect immunoperoxidase in DKA brains as described in Materials and Methods section. Hippocampal neurons exhibit low TIMP1 staining (A). In addition, reduced staining of TIMP1 was found in cells present in the blood vessels (B, C). Some intravascular TIMP1+ cells had morphology typical for neutrophils (C, arrows), while others for lymphocytes (C, arrowhead). Controls of Immunoperoxidase were negative (D). Magnification: x400 (in A, C and D) and x200 (in B).

Article Snippet: Sections were preincubated for 30 min with normal goat serum then with a rabbit monoclonal antibody (mAb) against human MMP9 (CST, Danvers, MA) or a rabbit polyclonal antibody against human TIMP1 (Millipore Sigma, Burlington, MA) over-night at 4°C in a humid chamber.

Techniques: Staining

Journal: Experimental and molecular pathology

Article Title: Intracerebral matrix metalloproteinase 9 in fatal diabetic ketoacidosis

doi: 10.1016/j.yexmp.2019.04.008

Figure Lengend Snippet: Our data suggest that during DKA, MMP9 is secreted by inflammatory cells and may be involved in BBB disruption, which leads to neutrophil infiltration into the brain parenchyma. In addition, activation of complement system with the assembly of terminal complement complex C5b-9 occurs both in plasma and in the brain tissue. It is possible that C5b-9 induces the expression of MMP9 particularly in hippocampal neurons, possibly by activating RGC-32. This increase in MMP9 expression may lead to hippocampal neuron dysfunction and degeneration. MMP9 intravascular and neuronal expression may also be facilitated also by the decreased expression of its natural inhibitor, TIMP1.

Article Snippet: Sections were preincubated for 30 min with normal goat serum then with a rabbit monoclonal antibody (mAb) against human MMP9 (CST, Danvers, MA) or a rabbit polyclonal antibody against human TIMP1 (Millipore Sigma, Burlington, MA) over-night at 4°C in a humid chamber.

Techniques: Activation Assay, Expressing